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RevoDx Набір для виділення ДНК ВПЛ DirEXT OneStep (Rt)
idil Bio з гордістю представляє революційний реагент Dirext NA Reagent, що пропонує блискавичне виділення ДНК ВПЛ менш ніж за 5 хвилин. Хоча...
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Updated website
We are pleased to announce the launch of the updated website of Med Bio Agro Laboratories!
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Med Bio Agro laboratories

ПЛР

    Med Bio Agro laboratories is a leading supplier of state-of-the-art equipment, reagents, consumables and service support in the fields of genomics, proteomics, cell technology, veterinary and food safety, clinical diagnostics, biopharma and forensics.

    We value long-term and strong relationships with our partners and offer qualified assistance and support at all stages of cooperation.

Training of laboratory personnel

We provide training in the basic methods of molecular diagnostics for laboratory staff (PCR, blotting, ELISA, electrophoresis, etc.).

Comprehensive laboratory equipment

We offer turnkey solutions to all issues, from the design stage to the commissioning of the laboratory.

Modern diagnostic equipment

A team of qualified specialists is always happy to help you with the optimal choice of state-of-the-art equipment based on your needs and requirements.

FAQ

Expiration dates of sets of reagents of production "Med Bio Agro laboratories"?

  1. Forez - 9 months.
  2. FLASH - 12 months (excluding Hepatitis and HIV kits).
  3. Rt - 6-12 months (it is necessary to specify the system).
  4. HLA - 6 months.
  5. KVM-9 months.

Expiration dates of test systems change, more detailed information can be found on the website or from the manager.

What are the formats of complete sets of reagents produced by "Med Bio Agro laboratories"?

The kits are digged into paraffin tubes (all but the reagent kits for genetic polymorphisms by real-time PCR).

Excavated sets:

  • striped (8 pieces of 0.2 ml) - Rt format;
  • single 0.5 ml - format forres / FLASH "Gin";
  • single 0.2 ml - FLASH format "Gin" and Rt.

When ordering sets with detection of FLASH and Rt it is necessary to specify the format of test tubes (single or striped)

More information can be found on the website.

In what format is the data obtained in the software "Med Bio Agro laboratories" stored?

* .Pdf Acrobat Reader

* .Jpg JPEG

* .Html HTML

* .Оdt Open Office

What is and in what cases use the analysis according to initial data?

The original data (mathematically unprocessed data, excluding the baseline and fitting) allow you to visually assess the growth of the fluorescence signal on each detection channel. Used to check questionable results (low fluorescence, irregular curve shape, etc.).

In which cases is the analysis of the results using the threshold method (Ct), and in which - the geometric method (Cp)?

By default, when analyzing optical measurements, RealTime_PCR uses the Geometric Method (Cp) - based on a mathematical analysis of the shape of the DNA accumulation curve during PCR.

Threshold method (Ct) - used in the case of non-standard situations, when the amplification curve differs significantly from the classical curve of DNA accumulation during PCR.

The threshold method may be recommended by other test system manufacturers. Based on drawing a "threshold" line parallel to the abscissa axis and determining the number of the reaction cycle (threshold cycle), on which the amplification curve for this tube crosses the boundary line.

Criteria of results (positive / negative / unreliable)?

"+" - in the sample there are fragments of the desired DNA (registered exponential signal growth for Specifics and registered / NOT registered signal growth for VC).

Specificity in standard programs (except genotyping - Analysis of polymorphisms, melting) for sets "Med Bio Agro laboratories" - is determined by the Fam channel.

Internal control (IR) in standard programs (except for genotyping - Analysis of polymorphisms, melting) for sets "Med Bio Agro laboratories" - is determined by the Hex channel.

Thus, a positive result is: Fam + / Hex +, or Fam + / Hex-

 "-" - in the sample there are no fragments of the desired DNA (no exponential signal growth for Specificity, in the case of using an internal control sample (VC) - registered exponential signal growth for VC).

Thus, the negative result is: Fam - / Hex +

"Sun" - an inaccurate result (when using VK - there is no exponential signal growth, both for Specifics and for VK).

Thus, the inaccurate result is: Fam - / Hex-

For which tests is a specialized report developed?

A specialized report is created for:

  • biocenoses (a set of reagents for the analysis of the biocenosis of the urogenital tract in women Femoflor, Androflor in men);
  • SNP, analysis of melting polymorphisms;
  • HPV (quantitative analysis and typing of human papilloma viruses - for a set of reagents Quantum);
  • multiplex analysis;
  • HLA typing;
  • analysis of AZF loci;
  • oncogenetics BRCA, CHECK2;
  • RHD, Gender;
  • Immunoquantex.

What types of analysis can be implemented using software "Med Bio Agro laboratories"?

Qualitative analysis - allows you to determine the presence or absence in the sample of the desired DNA fragment.

  1. Quantitative with standards.
  2. Analysis of polymorphisms, melting (for human genotyping).
  3. Multiplex analysis (TNC-complex, HerpesComplex, UMC-complex).
  4. Biocenosis (quantitative analysis of the biocenosis of the urogenital tract in women Femoflor, Androflor in men).
  5. HPV (for detection, typing and quantitative analysis of human papilloma viruses: sets of reagents Quantum).
  6. HMD (to identify genetically modified sources, determine their number (according to standards: 0.1%, 1%, 5%).
  7. HLA DR, HLA DQ (for HLA analysis).
  8. AZF (analysis of male infertility factors - azoospermia factor).
  9. RHD, Gender (Prenatal diagnosis).
  10. Relative - allows you to estimate the amount of the desired DNA fragment in the test samples relative to the "base" tube.
  11. Analysis of polymorphisms, 2 dyes - analysis of genetic polymorphisms, such as SNP, using two different fluorophores in one test tube.

How can I get / update software and ini files?

Software and ini-files can be obtained / updated:

  • on the CD-disk enclosed in the instruction to devices at delivery of the equipment;
  • on the website www.dna-technology.ua, section Support - Software for hardware (Software for hardware) and Files with test parameters for connection to the program (ini-files).
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